Trans-Hi™ is a superior transfection reagent capable to compete against the leading products on the market. It is based on a proprietary polymer technology. It forms stable nanoparticles with DNA and rapidly releases the DNA molecules upon endocytosis into the cytoplasm. It is a broad spectrum transfection regent offering superior and reproducible transfection efficiency on the common cells such as Hela, CHO, HEK293 and many hard-to-transfect mammalian cells with minimal toxicity. Trans-Hi™ is very cost-efficient and 1.0mL is sufficient for over 660 transfections in 24 well plates.
Key Features for Trans-HiTM
- Excellent transfection for single DNA transfection or multi DNA co-transfection
- Rapid onset and long-lasting transfection
- Suitable for very long DNAs
- Simple and easy to use transfection procedure
- Robust and reproducible transfection
- High level of recombinant protein production
- Very cost efficient
- Long shelf-life: 12 months
Specifications:
| Product type: | In vitro cell transfection reagent | |
| Applications | Plasmid transfection, protein overexpression | |
| Technique: | Polymer-based DNA nanoparticles | |
| Cell type: | Established cell lines, primary cells, hard-to-transfect cells Adherent cells and suspension cells | |
| Sample type: | Plasmid DNA | |
| Serum compatibility: | Yes | |
| Product size: | 0.5, 1.0 and 10x1.0 mL | |
| Number of transfections | Up to 660 in 24-well plates with 1.0mL reagent up to 330 transfections in 6-well plates | |
| Shelf-life: | 12 months or longer when store refrigerated (2-8°C) | |
| Shipping condition: | Room temperature |
Protocols
Trans-Hi™General Protocol For In Vitro Transfection in Mammalian Cells 
Trans-Hi™ - Protocol for Transfecting 293 and CHO Suspension Cells
Trans-Hi™ - Protocol for Generation of rAAV
Trans-Hi™ -Technical Tips For Good In Vitro Transfection
Comparison study of In vitro plasmid DNA transfection of Trans-HiTM and Lipofectamine® 2000 in HEK293T cells with GFP vector using the manufacture recommended reagent/DNA ratio of 3:1 (µL/µg) and 0.5µg DNA per well used for both products in 24 well plate 30 hours post transfection.
Trans-Hi™/DNA ratio (µL/µg) (HEK293T)
Study of the transfection efficiency as a function of transfection reagent/DNA ratio indicating optimal tranfection efficiency achieved at Trans-Hi™/DNA ratio of 3:1 (µL/µg) (HEK293T cells in 24 well plate 30 hours post transfection, 0.5ug DNA per well).
Head-to-head comparison Trans-Hi™ against Lipofectamine 2000 HEK293A cells with GFP vector (reagent/DNA ratio of 3:1(µL/µg, 0.5ug per well), 24 well plate 48 hours post transfection).
| Trans-Hi™ | Lipofectamine-2000 | |||||
| Time | Day 1 | Day 2 | Day 3 | Day 1 | Day 2 | Day 3 |
| HEK293A (%) | 79.5 | 93.4 | 93.3 | 75.7 | 89.4 | 50.6 |
Comparison study of In vitro plasmid DNA transfection of Trans-Hi™ and Lipofectamine® 2000 in Hela cells with GFP vector ((reagent/DNA ratio of 3:1(µL/µg, 0.5ug per well), 24 well plate 48 hours post transfection).
Broad Transfection Spectrum for Mammalian Cell Types
|
Cell Lines |
Efficiency (% GFP) |
|
COS-7 |
85 - 90% |
|
Hela |
85 - 90% |
|
HEK-293T |
85-90% |
|
NIH 3T3 |
75% |
|
A549 |
75% |
|
Hep3D |
65-75% |
|
SHEP |
65-70% |
|
CaCo2 |
60% |
|
HepG2 |
70% |
|
SKOV3 |
65% |
|
MCF-7 |
65-70% |
|
3T3-442A |
35-40% |
|
CHO |
80-90% |
