Fluorescent DOPC/CHOL Liposomes labeled with Dil (100nm)
High quality LUV liposomes labeled with Dil are ready to use. The liposomes are prepared by the extrusion method to 100nm with very tight particle size distribution. The product is sterile filtered and filled in sterile vials guaranteed to be stable for 6 months under recommended storage conditions.
Specifications
Lipid composition: DOPC/CHOL (54:45 mol/mol)
Lipid concentration: 50 mM (50-55 mM)
DiI: 0.5mM (0.47mg/mL)
Mean particle size: 100 nm (90-120 nm)
Hydration buffer: 10% sucrose, 20mM HEPES, pH 7.3 ± 0.2
Storage: in refrigerator (2 - 8 degree C)
Shelf-life: 6 months guaranteed for unopened vials
DOPC: 1,2-dioleoyl-sn-glycero-3-phosphocholine
CHOL: cholesterol
'DiI': DiIC18(3) (Thermofisher D282) is a lipophilic membrane dye. It is weakly fluorescent until incorporated into liposome membranes. This orange—red-fluorescent dye, which is spectrally similar to tetramethylrhodamine, is often used as a long-term tracer for neuronal and other cells.
Ex/Em: 550nm/570nm
CAS Name/Number: ('DiI'; DiIC18(3))) (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate, CAS 41085-99-8
