Fluorescent DOPC/CHOL Liposomes labeled with Fluorescein DHPE (100nm)
High quality LUV liposomes labeled with fluorescein DHPE are ready to use. The liposomes are prepared by the extrusion method to 100nm with very tight particle size distribution. The product is sterile filtered and filled in sterile vials guaranteed to be stable for 6 months when stored in refrigerator. The product is sterile filtered and filled in sterile vials guaranteed to be stable for 6 months when stored in refrigerator. The phospholipid, fluorescein DHPE is labeled on the head group with the green-fluorescent pH-sensitive fluorophore, fluorescein (ex/em ~496/519 nm).
For more information on the dye, see Thermofisher F362
Specifications
Product code: F60103F-F
Volume: 1.0mL
Lipid composition: DOPC/CHOL/F-DHPE (54:45:1 mol/mol)
Lipid concentration: 50 mM (50-55 mM)
Fluorscein DHPE: 0.5 mM (0.59 mg/mL)
Mean particle size (Z-average by Zetasizer): 100 nm (90-120 nm)
Hydration buffer: 10% sucrose, 20mM HEPES, pH 7.5 ± 0.2
Storage: in refrigerator (2 - 8 degree C)
Stability: 6 month for unopened vials, protect from light
DOPC: 1,2-dioleoyl-sn-glycero-3-phosphocholine
CHOL: cholesterol
Fluorescein DHPE: N-(fluorescein-5-thiocarbamoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (Thermofisher F362)