Fluorescent DOPC/CHOL Liposomes labeled with Rhodamine DHPE (200nm)
High quality LUV liposomes labeled with Rhodamine DHPE (Rh-DHPE) are ready to use. The liposomes are prepared by extrusion to 200-250nm with tight particle size distribution. The product is sterile filtered through 0.45um filters and filled in sterile vials guaranteed for 3 months shelf-life properly stored in refrigerator. Rh--DHPE is a phospholipid labeled on the head group with the red-fluorescent rhodamine B fluorophore with excitation/emission maxima ~560/580 nm. For more information on the dye, see Invitrogen L-1392.
Specifications
Product code: F60103F2-R
Lipid composition: DOPC/CHOL/Rh-DHPE (54:45:1 mol/mol)
Lipid concentration: 50 mM (50-55 mM)
Rh-DHPE: 0.5mM (0.67mg/mL)
Mean particle size: 230 nm (200-250 nm, intensity weighted lognormal per DSL)
Particle size distribution: 80-100nm (half-width)
Hydration buffer: 10% sucrose, 20mM HEPES, pH 7.5 ± 0.2
Storage: in refrigerator (2 - 8 degree C)
Shelf-life: 3 month for unopened vials, protect from light
DOPC: 1,2-dioleoyl-sn-glycero-3-phosphocholine
CHOL: cholesterol
Rhodamine DHPE: Lissamine™ rhodamine B 1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (Invitrogen L-1392)