Fluorescent DOPC/CHOL Liposomes labeled with Fluorescein DHPE (300nm)
High quality 300nm LUV liposomes labeled with fluorescein DHPE are ready to use. The liposomes are prepared by the extrusion method to 300nm with tight particle size distribution. The product is semisterile filtered through 0.45um filters and filled in autoclaved vials guaranteed to be stable for 3 months stored in refrigerator. The phospholipid, fluorescein DHPE is labeled on the head group with the green-fluorescent pH-sensitive fluorophore, fluorescein (excitation/emission maxima ~496/519 nm).
For more information on the dye, see Thermofisher F362
Specifications
Product code: F60103F3-F
Lipid composition: DOPC/CHOL/F-DHPE (54:45:1 mol/mol)
Lipid concentration: 50 mM (50-55 mM)
Fluorscein DHPE: 0.5 mM (0.59 mg/mL)
Mean particle size (z-average by Zetasizer) 280 nm (250-320 nm)
Hydration buffer: 10% sucrose, 20mM HEPES, pH 7.5 ± 0.2
Storage: in refrigerator (2 - 8 degree C)
Shelf-life: 3 month for unopened vials, protect from light
DOPC: 1,2-dioleoyl-sn-glycero-3-phosphocholine
CHOL: cholesterol
Fluorescein DHPE:N-(fluorescein-5-thiocarbamoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt (Thermofisher F362)