Trans-Hi™ - In vitro Transfection Reagent

$229.00
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SKU:
F90101TH-10
Availability:
1day

Trans-Hi™  is a superior transfection reagent capable to compete against the leading products on the market. It is based on a proprietary polymer technology. It forms stable nanoparticles with DNA and rapidly releases the DNA molecules upon endocytosis into the cytoplasm. It is a broad spectrum transfection regent offering superior and reproducible transfection efficiency on the common cells such as Hela, CHO, HEK293 and many hard-to-transfect mammalian cells with minimal toxicity. Trans-Hi™ is very cost-efficient and 1.0mL is sufficient for over 660 transfections in 24 well plates.

 

Key Features for Trans-HiTM

  • Excellent transfection for single DNA transfection or multi DNA co-transfection
  • Rapid onset and long-lasting transfection
  • Suitable for very long DNAs
  • Simple and easy to use transfection procedure
  • Robust and reproducible transfection
  • High level of recombinant protein production
  • Very cost efficient
  • Long shelf-life: 12 months

 

Specifications:

  Product type: In vitro cell transfection reagent
  Applications Plasmid transfection, protein overexpression
  Technique:  Polymer-based DNA nanoparticles 
  Cell type: Established cell lines, primary cells, hard-to-transfect cells                   Adherent cells and suspension cells
  Sample type: Plasmid DNA
  Serum compatibility:  Yes
  Product size: 0.5, 1.0 and 10x1.0 mL
  Number of transfections Up to 660 in 24-well plates with 1.0mL reagent                                           up to 330 transfections in 6-well plates
  Shelf-life: 12 months or longer when store refrigerated (2-8°C)
  Shipping condition: Room temperature

 

Protocols 

     Trans-Hi™General Protocol For In Vitro Transfection in Mammalian Cells 

     Trans-Hi™ - Protocol for Transfecting 293 and CHO Suspension Cells 

     Trans-Hi™ - Protocol for Generation of rAAV 

     Trans-Hi™ -Technical Tips For Good In Vitro Transfection 

 

trans-hi-lipofectamine-compare.jpg

Comparison study of In vitro plasmid DNA transfection of Trans-HiTM and Lipofectamine® 2000 in HEK293T cells  with GFP vector using the manufacture recommended reagent/DNA ratio of 3:1 (µL/µg) and 0.5µg DNA per well used for both products in 24 well plate 30 hours post transfection.

 

Trans-Hi™/DNA ratio (µL/µg) (HEK293T)

trans-hi-ratio.jpg

Study of the transfection efficiency as a function of transfection reagent/DNA ratio indicating optimal tranfection efficiency achieved at Trans-Hi™/DNA ratio of 3:1 (µL/µg) (HEK293T cells in 24 well plate 30 hours post transfection, 0.5ug DNA per well).

 

hek293a-042116.png

Head-to-head comparison Trans-Hi™ against Lipofectamine 2000 HEK293A cells with GFP vector (reagent/DNA ratio of 3:1(µL/µg, 0.5ug per well), 24 well plate 48 hours post transfection).

 

  Trans-Hi™ Lipofectamine-2000
Time Day 1 Day 2 Day 3 Day 1 Day 2 Day 3
HEK293A (%) 79.5 93.4 93.3 75.7 89.4 50.6

 

hela-042916c.jpg

Comparison study of In vitro plasmid DNA transfection of Trans-Hi™ and Lipofectamine® 2000 in Hela  cells with GFP vector ((reagent/DNA ratio of 3:1(µL/µg, 0.5ug per well), 24 well plate 48 hours post transfection).

 

Broad Transfection Spectrum for Mammalian Cell Types

Cell Lines

Efficiency (% GFP)

COS-7

85 - 90%

Hela

85 - 90%

HEK-293T

85-90%

NIH 3T3

75%

A549

75%

Hep3D

65-75%

SHEP

65-70%

CaCo2

60%

HepG2

70%

SKOV3

65%

MCF-7

65-70%

3T3-442A

35-40%

CHO

80-90%