HSPC/CHOL/mPEG2000-DSPE (56.2:38.5:5.3) Liposomes with Ammonium Sulfate Gradient (100nm)
HSPC/CHOL/mPEG2000-DSPE (56.2/38.5/5.3 mol/mol) liposomes with ammonium gradient is the original lipid copmposition used for the commercial product Doxil(R) (STEALTH liposomal doxorubicin HCl). This lipid composition is a formulator's choice in pharmaceutical/biotech industry for both formulation feasibility studies and liposomal product development. HSPC is essentially equivalent to DSPC (HSPC contains about 85 % DSPC and 15% DPPC). The main advantages for using HSPC are its lower cost and availability of GMP quality raw materials. HSPC material used at FormuMax is from one of the world’s premier lipid suppliers.
This liposomes has the same lipid composition as used in the commercial STEALTH liposomes, DOXIL® (doxorubicin HCl liposome injection). Note the larger liposome particle size of 100nm compared to 85nm for Doxil®. If you want an ammomium liposmes more comparable to Doxil®, please see F20204AS.
Product code: F20204AB
Lipid composition: HSPC/CHOL/mPEG2000-DSPE (56.2:38.5:5.3 mol/mol)
Mean particle size: 100 nm (95 - 120 nm)
Lipid concentration: 60 (60 -70mM)
Hydration buffer (loading battery): 250 mM ammonium sulfate
External solution: 10% sucrose, 20mM sodium PO4, pH 6.5 ± 0.2
Shelf-life: 6 months
Storage: 2-8 °C, do not freeze
HSPC: hydrogenated phosphatidylcholine (Soy)
mPEG2000-DSPE: 18:0 PEG2000 PE:1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt)