Fluorescent DOPC/CHOL/mPEG-DSPE Liposomes labeled with Dil (100nm)
High quality LUV liposomes labeled with Dil are ready to use. The liposomes are prepared by the extrusion method to 100nm with very tight particle size distribution. The product is sterile filtered and filled in sterile vials guaranteed to be stable for 6 months when stored in refrigerator.
Lipid composition: DOPC/CHOL/mPEG2000-DSPE (50:45:5 mol/mol)
Lipid concentration: 50 mM (50-55 mM)
DiI: 0.5mM (0.47mg/mL)
Mean particle size: 100nm (85-110nm)
Hydration buffer: 10% sucrose, 20mM HEPES, pH 7.3 ± 0.2
Storage: in refrigerator (2 - 8 degree C)
Shelf-life: 6 months guaranteed for unopened vials
DiI; DiIC18(3) (Invitrogen V22889) is a lipophilic membrane dye. It is weakly fluorescent until incorporated into liposome membranes. This orange—red-fluorescent dye, which is spectrally similar to tetramethylrhodamine, is often used as a long-term tracer for neuronal and other cells.
CAS Name/Number: 3H-Indolium, 2-[3-(1,3-dihydro-3,3-dimethyl-1-octadecyl-2H-indol-2-ylidene)-1-propenyl]-3,3-dimethyl 1-octadecyl-, perchlorate/ 41085-99-8
mPEG2000-DSPE: 18:0 PEG2000 PE:1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (ammonium salt)