100nm DOPC/CHOL Liposomes labeled with DiO

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Fluorescent DOPC/CHOL Liposomes labeled with DiO (100nm)

The fluorescent control liposomes contain the green lipophilic dye DiO incorporated in the bilayers. It is used for tracking the cellular uptake of the liposomes by common methods like confocal, FACS, etc.  

The green fluorescent, lipophilic carbocyanine DiOC18(3) is widely used as a lipophilic tracer. It is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments.



Lipid composition: DOPC/CHOL (54:45 mol/mol)

Lipid concentration: 50 mM (50-55 mM)

Mean particle size: 100 nm (90-120 nm)

Hydration buffer: 10% sucrose, 20mM HEPES, pH 7.3 ± 0.2

Dye: 0.5 mM (0.44 mg/mL)

Stability: 6 months for unopened vials

Storage: 2-8 ºC

'DiO';  DiOC18(3) (3,3'-Dioctadecyloxacarbocyanine Perchlorate)  (Invitrogen D-275) 

Ex/Em: 484/501 nm

CAS Number: 34215-57-1





DOPC: 1,2-dioleoyl-sn-glycero-3-phosphocholine

CHOL: cholesterol



Do you want the same dye in different liposomes, please contact us for a quote.

rhodamine DHPE