100nm DOPC/CHOL Liposomes labeled with DiR

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Fluorescent DOPC/CHOL Liposomes labeled with DiR (100nm)

The fluorescent control liposomes contain the lipophilic Near IR dye DiR incorporated in the bilayers. It is used for tracking the cellular uptake of the liposomes in vitro and in vivo by common methods like confocal, FACS, NIR imaging etc.  The near IR fluorescent, lipophilic carbocyanine DiOC18(7) ("DiR") is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. The sulfonate groups incorporated into this DiI analog improves water solubility. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. 


Lipid composition: DOPC/CHOL (54:45 mol/mol)

Lipid concentration: 50 mM (50-55 mM)

Mean particle size: 100 nm (90-120 nm)

Hydration buffer: 10% sucrose, 20mM HEPES, pH 7.3 ± 0.2

DiR: 0.5 mM (0.51mg/mL)

Stability: 6 months for unopened vials

Storage: 2-8 ºC

'DiR';  (Invitrogen D-12731)   DiIC18(7) (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindotricarbocyanine Iodide)

Ex/Em: 750/780 nm

CAS Number: n/a


DOPC: 1,2-dioleoyl-sn-glycero-3-phosphocholine

CHOL: cholesterol

rhodamine DHPE